Figure_3.tif (1.34 MB)
RT-PCR of IL-24 and its receptor subunits.
figure
posted on 2013-02-21, 11:25 authored by Stephanie Kreis, Demetra Philippidou, Christiane Margue, Catherine Rolvering, Claude Haan, Laure Dumoutier, Jean-Christophe Renauld, Iris BehrmannEight different melanoma cell lines, primary NHEM and HaCaT cells were grown on Petri dishes to a confluence of 80–90% before cells were harvested. Total RNA was extracted, reverse transcribed and cDNA amounts equivalent to 100 ng RNA were used as input material for amplifications of IL-24, and the receptor subunits IL-20R1, -20R2, and -22R. To control for equal starting amounts and loading, actin was amplified. Size markers (bp) are indicated on the left.