Figure_2.tif (1.34 MB)
RNAi–mediated FISH–based screen of Drosophila cells for heterochromatic pairing factors.
figure
posted on 2012-05-10, 00:07 authored by Eric F. Joyce, Benjamin R. Williams, Tiao Xie, C.-ting Wua, Experimental design with representative image before and after automated identification of nuclei and FISH signals. The screen averages for signals per nucleus obtained with probes targeting 359 and dodeca. b, Rank-order plot of each dsRNA in the primary screen, where negative z-scores indicate a reduction in paired nuclei (corresponding to candidate pairing promoters) and positive z-scores indicate an increase in paired nuclei (corresponding to candidate anti-pairers). c, Functional classifications of the candidate pairing promoters and anti-pairers.