Quasispecies breadth is lower in relapser than nonresponder sequences.

Nonresponder and relapser sequences were analyzed using Phred and positions at which the dominant nucleotide was present at <80% frequency were determined. A) Number of positions of quasispecies diversity in nonresponders and relapsers in pre- and post-therapy samples. B) Proportion of positions of mixed nucleotides in post-therapy samples relative to pre-therapy samples. One nonresponder (3.22) and one relapser (10.00) are not shown. The relapser is the same as was removed from Figure 3A.