Quantitative Real Time-PCR of the 205B allele in Pk1(B+)1+ and Pk1(B-)1- ring and trophozoite stage parasites.

Copy number profiles showing that SICAvar transcripts representing the 205 kDa SICA protein that is expressed by the Pk1(B+)1+ parasites are most abundant in the ring stage (R) and reduced in the trophozoite stage (T), while greatly reduced in comparable parasite samples from the Pk1(B-)1- clone. The conserved exon 12 based Cyto5 primer was used to initiate the experiment.