Quantitative PCR of mutant and wildtype hypertrophic zones.

qPCR with primers specific for (A) Agc1, (B) Ctgf, (C) Matn1, (D) Creld2, (E) Derl3, (F) Ero1l, (G) Fgf21, (H) Steap1, and (I) p58IPK on cDNA derived from Wt, Xbp1CartΔEx2, ColXN617K and C/X hypertrophic zone aRNA. Plots depict mean fold differences with standard deviation from the mean, N = 3, statistical significance was determined using Student’s t test, ** p < 0.01, *** p < 0.001, **** p < 0.0001.