Purification of cellular interaction partner of influenza strains A/Beijing/501/2009(H1N1) NS1.

A, The purified protein complexes were detected by SDS-PAGE. Left panel: the protein complexes purified from A549 cells transfected with pnTAP-NS1 plasmids. Right panel: the protein complexes purified from A549 cells transfected with pnTAP vector. M represents protein marker. The bait protein bands and its cellular interaction proteins identified by mass spectrometry are indicated by an arrow and an asterisk, respectively. B, The purified protein complexes were detected by immunoblotting with antibodies against CBP. Left panel: the protein complexes purified from A549 cells transfected with pnTAP-NS1 plasmids. Right panel: the protein complexes purified from A549 cells transfected with pnTAP vector.