Purification of SAVSBPM18.
Secreted SAVSBPM18 produced from WB800[pSAVSBPM18] was affinity purified using biotin-agarose. Fractions were analyzed by SDS-PAGE. M: Molecular weight markers. The numbers shown on the left represent the molecular mass expressed in kDa; CS: culture supernatant; FT: Flow-through fraction; W: wash fraction; E1–E6: elution fractions. Arrowhead marks the position of SAVSBPM18.