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Purification of SAVSBPM18.

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posted on 2013-07-16, 03:16 authored by Sau-Ching Wu, Sui-Lam Wong

Secreted SAVSBPM18 produced from WB800[pSAVSBPM18] was affinity purified using biotin-agarose. Fractions were analyzed by SDS-PAGE. M: Molecular weight markers. The numbers shown on the left represent the molecular mass expressed in kDa; CS: culture supernatant; FT: Flow-through fraction; W: wash fraction; E1–E6: elution fractions. Arrowhead marks the position of SAVSBPM18.

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    BiochemistryproteinsProtein interactionsprotein structureRecombinant proteinsBiomacromolecule-ligand interactionsbiotechnologymicrobiologyApplied microbiologyproteomicsProtein engineeringSynthetic peptideSynthetic BiologychromatographyAffinity chromatographySize exclusion chromatography

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