Purification of Eut compartments.

<p>(<b>A</b>) Silver stained SDS-PAGE gel showing purification of (lane 1) Eut BMCs from <i>S. enterica</i> cells harboring EutC<sup>1–19</sup>-EGFP, (lane 2) recombinant EutSMNLK BMCs, and (lane 3) recombinant EutS BMCs from <i>E. coli</i> C2566 cells co-expressing EutC<sup>1–19</sup>-EGFP. Calculated protein sizes are as follows: EutS (11.6 kDa), EutM (9.8 kDa), EutN (10.4 kDa), EutL (22.7 kDa), EutK (17.5 kDa), EutC<sup>1–19</sup>-EGFP (29.1 kDa). (<b>B</b>) Transmission electron micrographs of isolated native and recombinant Eut compartments. From left to right: Eut BMCs from <i>S. enterica</i>, EutSMNLK shells from <i>E. coli</i> C2566, EutS shells from <i>E. coli</i> C2566. (Scale bar: 100 nm).</p>

Keyword(s)

eut

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