Purification of Eut compartments.
(A) Silver stained SDS-PAGE gel showing purification of (lane 1) Eut BMCs from S. enterica cells harboring EutC1–19-EGFP, (lane 2) recombinant EutSMNLK BMCs, and (lane 3) recombinant EutS BMCs from E. coli C2566 cells co-expressing EutC1–19-EGFP. Calculated protein sizes are as follows: EutS (11.6 kDa), EutM (9.8 kDa), EutN (10.4 kDa), EutL (22.7 kDa), EutK (17.5 kDa), EutC1–19-EGFP (29.1 kDa). (B) Transmission electron micrographs of isolated native and recombinant Eut compartments. From left to right: Eut BMCs from S. enterica, EutSMNLK shells from E. coli C2566, EutS shells from E. coli C2566. (Scale bar: 100 nm).