Protein levels of Bax, Bcl-2 and PCNA were detected by Western blot (Figure 4A) and quantified individually Bax (Figure 4B), Bcl-2 (Figure 4C) and PCNA (Figure 4D).

Figure 4B–D: X axis represents the relative gray values of Western blot bands of Bcl-2, Bax or PCNA compared to that of house keeping protein beta-actin.