Protection of mice from lung viremia.
2013-02-21T02:32:02Z (GMT) by
<p>Groups of CD1 mice were immunized twice with five-fold serial dilutions of pandemic H1N1 (H1N1 A/California/7/2009) whole virus vaccine, before being challenged intranasally with 10<sup>5</sup> TCID<sub>50</sub>. Lungs were harvested at day three after challenge, and virus titers determined as described (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009349#s2" target="_blank">Methods</a>). Lack of detection of virus in lungs was considered indicative of protection.</p>