Figure_2.tif (522.4 kB)
Production of 11-cis retinol by Lamprey RPE65 in HEK293F cells.
figure
posted on 2012-11-27, 01:44 authored by Eugenia Poliakov, Alexander N. Gubin, Olivia Stearn, Yan Li, Maria Mercedes Campos, Susan Gentleman, Igor B. Rogozin, T. Michael RedmondA: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 and bovine LRAT (blue trace). B: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 with Lamprey LRAT (red trace) or only Lamprey LRAT (green trace).