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Production and biochemical characterization of HCV E2e.
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posted on 2010-02-19, 00:35 authored by Thomas Krey, Jacques d'Alayer, Carlos M. Kikuti, Aure Saulnier, Laurence Damier-Piolle, Isabelle Petitpas, Daniel X. Johansson, Rajiv G. Tawar, Bruno Baron, Bruno Robert, Patrick England, Mats A. A. Persson, Annette Martin, Félix A. ReyA) Schematic diagram of the HCV genome region coding for the structural proteins and the constructs used to make stable S2 cell transfectants expressing E2e. MT: inducible metallothionin promoter, BiP: Drosophila BiP Signal peptide, EK: enterokinase cleavage site, ST: Strep-Tag. B) Elution profile of E2e from the HCV isolates indicated from an Sdx200 size exclusion column. Bottom panels: Non-reducing SDS-PAGE analysis of the eluted fractions. Arrows indicate multimeric (A), dimeric (D) and monomeric (M) forms of the protein.
