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Primers and probes for real-time polymerase chain reaction (PCR) and reaction conditions.

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posted on 2014-03-24, 03:12 authored by Min-Nan Tseng, Chia-Ling Chung, Shean-Shong Tzean

*PCR conditions: denature at 94°C for 3 min, 1 cycle; denature at 94°C for 1 min, anneal at 57°C for 1 min, and extension at 72°C for 2 min, 30 cycles; elongation at 72°C for 5 min, 1 cycle.

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