Presynaptic expression of DISC1ΔC alters the kinetics of evoked glutamate release and inhibits synchronous glutamate release.
A) Normalized EPSCs recorded from an untransfected neuron and evoked from presynaptic terminals expressing ChR2 and either dsRed (control), DISC1ΔC, wtDISC1 or D1 RNAi. B) Summary graphs showing the mean ± SEM of four different kinetic measures of EPSCs evoked from presynaptic terminals expressing ChR2 and either dsRed (control), DISC1ΔC, wtDISC1 or D1 RNAi. C) Two representative recordings from a neuron in control or DISC1ΔC conditions showing 10 consecutive traces that are normalized and overlaid (top traces). The average trial-to-trial variance for the traces above is displayed over the duration of the waveform (bottom trace). D) A histogram depicting the average trial-to-trial variance for all neurons in each condition (bin = 0.001 A2). E) The ratio of the average trial-to-trial variance for each condition (black trace) is displayed over the duration of the EPSC waveform. The dotted blue line indicates the threshold for statistical significance (p<0.05; F-test). A representative DISC1ΔC EPSC (red trace) is overlaid to emphasize the aspects of the EPSC waveform that produce the most variance.