Presence of PrtV in OMVs from V. cholerae strain C6706.

To detect the PrtV and the OmpU proteins in the density gradient fractions, immunoblot analyses were performed using polyclonal anti-PrtV and anti-OmpU antisera, respectively. (A) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the wild type V. cholerae strain C6706. (B) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the prtV mutant. (C) Immunobot detection of PrtV in the whole cell lysate (lane 1), culture supernatant before ultracentrifugation (lane 2), supernatant after the removal of OMVs (lane 3), and OMV sample (lane 4).