Predictions filtered by conservation, accessibility, or both are more downregulated than non-filtered predictions.

Mean log₂ fold changes in protein expression are shown for the targets of five highly conserved miRNAs, predicted using PACCMIT with different filters. The log₂ fold changes are taken from Selbach et al. [7]. Statistical significance is given by the one-sided Wilcoxon rank sum test (*P≤0.05, **P≤0.01, ***P≤0.001). P-values>0.05 are not indicated. Error bars indicate standard errors of the mean.