Por secretion system (PorSS)-dependent cell surface localization of HBP35.
(A) Immunoblot analysis of P. gingivalis PorSS-deficient mutants with anti-HBP35 (upper) and anti-A-LPS (lower). (B) Dot blot analysis of P. gingivalis PorSS-deficient mutants with anti-HBP35 (upper), anti-A-LPS (middle) and anti-PtpA (lower). (C) Immunoblot analysis of the periplasmic fractions of PorSS-deficient mutants. Periplasmic fractions were subjected to SDS-PAGE and the resulting gel was stained with Coomassie Brilliant Blue (CBB) (left), or was immunoblotted with anti-HBP35 (middle) and anti-TprA (right). Asterisks indicate nonspecific cross-reactive protein bands.