Phylogenetic relatedness of OnOrs1-6 to the Lepidoptera sex pheromone receptor subfamily, neighbor-joining (corrected distance) tree.

Bootstrap values are presented as a percentage of n = 1000 replicates at significant branch points. The tree is rooted with lepidopteran orthologs of DmOr83b. The responses of receptors that have been functionally characterized are indicated by numbers corresponding to the 15 pheromone compounds listed, bolded numbers indicate the strongest response. Bm, Bombyx mori; Di, Diaphania indica; Ep, Epiphyas postvittana; Hv; Heliothis virescens; Msex, Manduca sexta; On, Ostrinia nubilalis; Px, Plutella xylostella; Msep, Mythimna separata. Superfamily taxonomies are delineated by vertical bars. ECB receptors reported in this study are bolded; OnOr1* was reported in [7] and is identical to OnOr5 in this study. Pheromone ligands: 1) E11–14:OH; E11-tetradecen-1-ol, 2) Z9–14:Al; Z9-tetradecenal, 3) Z9–14:OAc; Z9-tetradecenyl acetate, 4) Z11–14:OAc; Z11-tetradecenyl acetate, 5) E11–14:OAc; E11-tetradecenyl acetate, 6) Z12–14:OAc; Z12-tetradecenyl acetate, 7) E12–14:OAc; E12-tetradecenyl acetate, 8) Z11–16:OH; Z11-hexadecen-1-ol, 9) Z9–16:Al; Z9-hexadecenal, 10) Z11–16:Al; Z11-hexadecenal,11) E11–16:Al; E11-hexadecenal, 12) Z11–16:OAc; Z11-hexadecenyl acetate, 13) E11–16:OAc; E11-hexadecenyl acetate, 14) E10, Z12–16:OH; E10,Z12-hexadecadien-1-ol, and 15) E10, Z12–16:Al; E10,Z12-hexadecadienal.