Phenotype of OsGA2ox5-overexpressing plants.

(A) Phenotype of WT (left) and dwarf OsGA2ox5-ox plants (right). 2-week-old water cultured seedlings were used for photograph; (B) Arrows indicate the boundary between the second leaf sheath and the blade of 5-day-old water cultured seedlings. Bar = 1 cm; (C) Longitudinal sections of the elongated regions of the second leaf sheath of WT (left) and OsGA2ox5-ox plants (right). Bar = 25 µm; (D) Quantitative measurement of the cell length of second leaf sheath in WT and OX (n = 20). Error bars show standard errors (SE). Asterisks indicated significant differences at P <0.01 compared with the wild type by Student’s t test; (E) Ectopic expression of OsGA2ox5 in Arabidopsis. Left is OsGA2ox5 transgenic plants and wide type Arabidopsis (Col) is on the right. Plants photographed are 4-weeks-old. Bar = 2.5 cm; (F) Expression level of OsOsGA2ox5 in transgenic rice; WT was used as a control; (G) Southern blotting analysis of transgenic plants. Restriction endonuclease Hind III was used to digest the genomic DNA from the leaf tissue. M, molecular marker; WT, wild type; L7, L12, L13, three transgenic lines.