Passive immunization with 9GlcNH2-Hla antibodies in a murine model of S. aureus pneumonia.

(a) Mice were vaccinated i.n with 20 µl of 9GlcNH2-Hla antisera or NRS 24 h and 4 h prior to infection with indicated S. aureus strains (4×108 CFU/mouse). Bacterial burden was recorded 24 h post-infection. Points indicate cfu/lung of individual mice, lines the group median; p values: Mann-Whitney test. (b) Kaplan–Meier survival curve of 4 week old FVB mice vaccinated i.n with 20 µl of 9GlcNH2-Hla antisera or NRS 24 h and 4 h prior to infection with indicated S. aureus strains (6×108 CFU/mouse). Survival was monitored at least twice a day over a 5 day period and data analyzed using the Log-rank test to derive the p values.