Partial CLUSTAL 2.1 multiple sequence alignment of publicly available Brugia malayi (Bm1_48165), Homo sapiens (HsSAH), Saccharomyces cerevisiae (ScSAH), and our cloned Brugia malayi (BmSAH) S-adenosyl homocysteinases, showing the “insert” missing from the publicly available nematode protein sequence.

The absence of the 27 amino acids in the publicly available Brugia sequence (Bm1_48165) could indicate a splice variant of the enzyme or simply a problem with the genome assembly.