Parasite morphology and global protein phosphorylation pattern of PKG inhibitor-treated P. falciparum schizonts.

(A) Immunofluorescent staining of DMSO/compound 1-treated WT schizonts using antibodies detecting (i) PfGAP45 [34], (ii) PfSUB1 [14] and (iii) PfAMA1 [23]. Representative images are shown for each staining together with parasite nuclei stained with DAPI. Bars ∼5 µM. (B) Metabolic labelling of phosphoproteins in P. falciparum schizonts. Autoradiographs of (i) 3D7 WT and (ii) gatekeeper mutant 3D7 PfPKG_T618Q schizonts, treated with ³²P-orthophosphate and DMSO (−) or compound 2 (+) prior to lysis, ÄKTA anion exchange chromatography (fractions 10–14 are shown) and separation by SDS-PAGE. Rectangular boxes highlight bands that show a differential signal following inhibitor-treatment in WT, but not PfPKG_T618Q schizonts.