Parasite load achieved using different vaccination strategies.

Parasite load in spleen (relative, fold more DNA copies than the calibrator) and parasite load in blood (parasites/mL) 20 weeks after Leishmania infantum challenge in hamsters vaccinated with KMPII, TRYP, LACK, and PAPLE22. C: Control (n = 6); PROT: Raw extracts of Trichoplusia ni larvae producing recombinant proteins (n = 6); DNA: Naked plasmid DNA (n = 6); DNA-PROT: Both vaccines combined in a prime-boost strategy (n = 5). Each sample was analyzed in triplicate.