Overview of the Arabidopsis growth experiment on soil.
Mutant lines are grown alongside their respective wildtype backgrounds on trays containing soil medium with different levels of dissolved MgSO4·7H2O. To control for environmental variation, each tray was divided in 8 sections, with a mutant line and its associated wildtype background line planted in alternating fashion. Yellow boxes highlight the sections with mutant plants within each flat; the other sections contain wildtype plants. For the comparison between cax1/cax3 and Col-0, 60 mM was not tested. The plants shown here are 3 weeks old and were harvested and analyzed after completing 4 weeks of growth. The experiment was repeated three times.