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Overview of affinity proteomics screening and study design.

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posted on 2014-04-17, 03:41 authored by Julie Bachmann, Florence Burté, Setia Pramana, Ianina Conte, Biobele J. Brown, Adebola E. Orimadegun, Wasiu A. Ajetunmobi, Nathaniel K. Afolabi, Francis Akinkunmi, Samuel Omokhodion, Felix O. Akinbami, Wuraola A. Shokunbi, Caroline Kampf, Yudi Pawitan, Mathias Uhlén, Olugbemiro Sodeinde, Jochen M. Schwenk, Mats Wahlgren, Delmiro Fernandez-Reyes, Peter Nilsson

(A) Schematic overview of the affinity proteomics approach using antibody suspension bead arrays. Plasma samples were biotinylated, antibodies were coupled to color-coded magnetic beads, and both were combined for analysis. Bead identity and captured plasma proteins were then detected using a flow cytometric analyzer. (B) Experimental design of study. Initial screening with 1,132 antibodies from targeted and blinded selections was performed in the discovery cohort (n = 356). Data from the patient groups were compared using univariate tests and multivariate penalized regression models. Identified single proteins and multi-component protein panels discriminating the 3 disease groups were validated in the verification cohort (n = 363).

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