Figure_4.tif (332.62 kB)
Overlapping peptide pools of latency-associated protein Rv3407 stimulate IFNγ-expressing CD4+ T cells after 7 days and two rounds of restimulation.
figure
posted on 2013-02-21, 05:56 authored by Sebastian D. Schuck, Henrik Mueller, Frank Kunitz, Albert Neher, Harald Hoffmann, Kees L. C. M. Franken, Dirk Repsilber, Tom H. M. Ottenhoff, Stefan H. E. Kaufmann, Marc JacobsenPBMC from six LTBI (A–F) were restimulated with 15-mer synthetic peptide pools of Rv3407 for 7 days including two rounds of in vitro restimulation. IFNγ-expressing CD4+ CD45RO+ T cells are shown for stimulation with peptide pools 1 to 6 (grey bars) and pools 7 to 11 (black bars). Each peptide is constituent of one pool within pools 1 to 6 and of one pool within pools 7 to 11. Peptides inducing the most prominent responses are indicated for donors A–D. The horizontal line indicates the threshold for positive responses (0.2%). Background values of non-stimulated controls were subtracted.