Nicotinamide Inhibits Ethanol-Induced Neurodegeneration

(A) Fluoro-Jade B staining 24 h after ethanol treatments.

(B) Quantification of Fluoro-Jade–B positive cells in the cingulate cortex (ANOVA F_2,13 = 25.541; p < 0.0001; n = 5–6 each treatment group), in the total CA1 region of hippocampus (ANOVA F_2,13 = 9.988; p = 0.0024; n = 5–6 each treatment group), and in the LDN of thalamus (ANOVA F_2,13 = 20.785; p < 0.0001; n = 5–6 each treatment group).

(C) Total number of NeuN positive cells were counted 2 wk after ethanol administration in the cingulate cortex (ANOVA F_2,12 = 9.894; p = 0.0029; n = 5 each treatment group), in the CA1 region of hippocampus (ANOVA F_2,12 = 7.289; p = 0.0085; n = 5 each treatment group) and in the LDN of thalamus (ANOVA F_2,12 = 5.551; p = 0.0196; n = 5 each treatment group). Values are shown as mean ± SD. Bonferroni correction for multiple comparison revealed a significant difference between ethanol treatment group and all other groups.

S, saline; E, ethanol; Etoh+Nic, ethanol + nicotinamide. Scale bars 200μm.