Figure_5.tif (436.25 kB)

NP expression in mammalian cells leads to downreglation of PKR and eIF2α phosphorylation.

figure

posted on 2011-06-15, 01:33 authored by Kulbhushan Sharma, Shashank Tripathi, Priya Ranjan, Purnima Kumar, Rebecca Garten, Varough Deyde, Jacqueline M. Katz, Nancy J. Cox, Renu B. Lal, Suryaprakash Sambhara, Sunil K. Lal

HEK293T cells were transfected with NP expressing plasmid (pcDNA3.1-NP) or control plasmid (pcDNA3.1). Cells were harvested at 36 hours post-transfection and cell lysates were subjected to western blotting analysis. A. Lane 1 of panel 2 shows significant downregulation of pPKR levels in NP transfected cells. Panel 1 shows NP expression level, whereas panel 3 shows equal loading trough β-Actin control. B. Figure shows graphical representation of relative pPKR levels as measured by western blotting followed by densitometric measurement in 3 independent experiments. Error bars represent standard deviation. C. Lane 1 of panel 2 shows significant downregulation of p-eIF2α levels in NP transfected cells. Panel 1 shows NP expression level, whereas panel 3 shows equal loading trough β-Actin control. D. Figure shows graphical representation of relative p-eIF2α levels as measured by western blotting followed by densitometric measurement in 3 independent experiments. Error bars represent standard deviation.