NMS profile of the interactions of Kid with CcdA proteins at different toxin-antitoxin ratios.

(A), Mass spectrum obtained at a Kid∶CcdA ratio of 2∶1. (B), Mass spectrum obtained at a Kid∶CcdA ratio of 1∶1.(C) Mass spectrum obtained at a Kid∶CcdA ratio of 1∶2. All the protein mixtures were performed in 100 mM ammonium acetate pH 6.7. The lowest protein concentration was 10 µM in all cases. CcdA protein is represented with maroon circles and Kid protein is represented with blue squares. Each complex found is represented with the appropriate combination of squares and circles.