NELF-E Expression in NELF-E[PB] germline clone embryos.

Western blots were done on 0–1 hour AED collections of wild-type and NELF-E[PB] GLC embryos to determine whether the transcripts expressed in the GLC embryos produced protein. The Western blot shown in this figure indicates reduced levels of NELF-E relative to α-tubulin in the NELF-E[PB] GLC embryos.