Myc overexpression in the adult increases mutation frequency and reduces lifespan at 25

°C. (A) Western analyses showing levels of Myc and the loading control γ-tubulin from four female adult heads of the genotypes lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as “ActTS>+”) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as “ActTS>Myc”). Adults were placed at 25°C for 10 days before Western analyses. (B) lacZ mutation frequency of females of genotype lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as “ActTS>+”) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as “ActTS>Myc”). Adults were placed at 25°C for 10 days before lacZ analyses. *indicates statistical significance of p<0.001 (student’s t-test). (C) Lifespan analyses at 25°C of females of the genotypes lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as “ActTS>+”) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as “ActTS>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test). (D) Lifespan analyses at 25°C of females of the genotypes lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-p35 (shown as “ActTS>p35”) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc, UAS-p35 (shown as “ActTS>Myc+p35”). These are statistically significantly different (p<0.001, Log-rank test).