MyD88 dependent but TLR independent IL-12 production from peritoneal macrophages stimulated by LAB.

(A) Peritoneal macrophages from wild type or TLR2-, 4-, 9- or MyD88-deficient mice were cultured with 1 µg/ml KW3110 for 24 hrs. IL-12p70 production was determined by ELISA. Data value is the mean ± SD of three mice. (B) KW3110, French pressed KW3110 or positive ligand for each TLRs were cultured with HEK 293 cells expressing indicated TLR. TLR stimulation was analyzed by assessing alkaline phosphatase activity (OD650 nm) which is under the control NF-κB activation. Data are representative of two independent experiment yielding same results. (C) Peritoneal macrophages from wild type mice were cultured with 1 µg/ml KW3110, FSL-1, LPS or 1 µM CpG. Cells were recovered at the indicated time points (0, 0.5, 8, 24 hr) and analyzed by Western blot for IRAK-1 and actin. Data are representative of two independent experiments.