Multiple sequence alignment on the protein data set.

Multiple sequence alignment was performed on 86 proteins in the data set using COBALT [55]. The resulting phylogenetic tree is visualized using Interactive tree of life (ITOL) [56, 57] (Blue: PBPs, Green: Ambler Class A, Dark Blue: Ambler Class C, Yellow: Ambler Class D, Orange: Ambler Class B. Same coloring scheme is used in the next figure.)