Mouse cerebral vascular morphology imaged by SRA and blood flow measured by LSCI.
(A) Cerebral vascular morphology imaged by SRA. (a) cerebral vascular morphology was obtained before middle cerebral artery occlusion (MCAO) in adult mice. CCA, ICA, PPA, PCA, MCA and ACA as well as their branches could be clearly detected. (b) SRA after MCAO showed that CCA, ICA, PPA and PCA could be detected while MCA was missing, suggesting that MCA was occluded. (c) SRA was performed 1 hour after reperfusion in heparin-treated mice, which showed CCA, ICA, PPA, PCA and MCA were well filled with contrast agent Ipamiro. (d) SRA was performed 1 hour after reperfusion in the heparin-free mice, which showed CCA, ICA, PPA and PCA could be detected while MCA was still undetectable. (B) CBF was measured by LSCI. (a) LSCI was recorded in normal mice showing that the CBF of ipsilateral hemisphere was normal, similar to that of the contralateral. (b) LSCI was recorded after MCAO showing that the CBF of the ipsilateral hemisphere decreased to 10% of the baseline. (c) LSCI was recorded 1 hour after reperfusion in heparin-treated mice showing that the CBF recovered to the baseline. (d) LSCI recorded 1 hour after reperfusion in the heparin-free mice showing that the CBF was still low. Arrows indicate that MCA was well filled with blood. Arrowheads indicate the location of MCA, which was undetectable. Broken Circle indicated the MCA territory in the cortex of ipsilateral hemisphere. Bar= 1 mm. Color bar: normalized relative CBF speed (0%~100%). ACA: anterior cerebral artery, CBF: cerebral blood flow, CCA: common carotid artery, ICA: internal carotid artery, LSCI: laser speckle contrast imaging, PCA: posterior cerebral artery, PPA: pterygopalatine artery, SRA: synchrotron radiation microangiography.