Monitoring of proteolytic activity in neutrophil extracellular traps.

A. Identification of an internally quenched fluorescent substrate that is hydrolyzed by NE, CG and PR3. B. Extracellular proteolytic activity was analyzed from 3 donor neutrophils (Donor 1, dark grey; Donor 2, black; Donor 3, light grey) following treatment with PMA, MNase or a combination of both. Proteolytic activity was measured using (K-Amc) PLGKQVEY(K-Dnp).