Molecular characterization of transgenic rice lines expressing AhSTS1.
(A) Southern blot analysis. Genomic DNA in lanes P and RS1 to RS22 were digested with BamHI (specific to the T-DNA region). The arrow indicates the fragment (1.2 kb) hybridized with the AhSTS1 cDNA probe. P, pSB2220 vector; N, non-transgenic wild-type Dongjin; lanes RS1 - RS22, representative transgenic Dongjin lines out of 129 T1 samples. (B) RT-PCR analysis. Total RNA from leaf samples of the same lines as in (A) was analyzed. OsUBQ5 was included as a PCR control.