Microarray analysis of miRNA expression changes in Müller cells post-ESMV treatment.
(A) Venn diagram of miRNA expression changes in ESMV-treated vs. control Müller cells at 8, 24, and 48 hours post-ESMV treatment. The expression of 25 miRNAs was altered at all three time points tested. The majority of the miRNA expression changes occurred at 48 hours post-ESMV, progressively increasing from 41 miRNAs at 8 hours, to 60 miRNAs at 24 hours, and 148 at 48 hours. (B) Heat map representation of hierarchal clustering of 16 samples based on 25 miRNA probes differentially regulated in ESMV-treated vs. control Müller cells at all times tested. (p<0.05, minimum 3-fold difference in expression). Each row represents a single sample, and each column-a single miRNA. The red or blue color represents relatively high or low expression, respectively. The two main groups of the dendrogram, labeled EXPT and CTR, separate treated vs. control groups of Müller cells.