Metabolomics profile by NMR spectroscopy.

(A) Control H460 cells or (B) cells treated for 24 h with 10 mM NaB were incubated in DMEM containing ¹³C-glucose. After the incubation, cells were harvested, and intact cells were analyzed by NMR spectroscopy. Spectra highlight the enrichment of the following ¹³C-containing informative metabolic intermediates: (1) Oxoglutaric acid; (2) Citric acid; (3) Fumaric acid; (4) L- Asparagine; (5) L-Glutamic acid; (6) L-Lysine; (7) L-Leucine; (8) 5-Methylcytidine; (9) 5-Methyldeoxycytidine; (10) Uridine; (11) Deoxyinosine; (12) Deoxyguanosine; (13) Coenzyme A; (14) 2-Acetolactate; (15) dGDP; (16) dGTP; (17) NAD; (18) NADPH; (19) NADP; (20) Phosphoribosyl pyrophosphate (PRPP); (21) Sedoheptulose 7-phosphate; (22) Xylulose 5-phosphate; (23) Fructose 6-phosphate; (24) Gluconolactone; (25) Cytidine triphosphate; (26) Cytidine monophosphate; (27) 2-Phospho-glyceric acid; (28) Glucose 6-phosphate; (29) 6-Phosphonoglucono-lactone; (30) Glyceric acid 1,3-biphosphate, (31) Malic acid; (32) 6-Phosphogluconic acid; (33) Erythrose 4-phosphate; (34) Ribulose 5-phosphate; (35) Acetoacetic acid; (36) L-Glutamine; (37) L-Cysteine; (38) L-lactate; (39) L-Alanine.