Male-Specific Amplification of TETY2 and CUL4BY STS Primers in Cat and Dog

A fragment of both genes has been PCR amplified with Y chromosome STS markers in matched cat and dog male and female genomic DNA samples. A 1-kilobase (kb) DNA ladder is shown to the left and right. PCR products are of the expected size in male DNAs, whereas no or nonspecific amplification is observed in female DNAs. The third lane in each set of primers (−) is a no-DNA control reaction. Dog ESTCO610012 is orthologous to cat TETY2.