Maintenance of s.tGlmS+p in S. typhimurium proliferating in tumor tissue.

(A) GlmS mutant (SKS1002, A) and parental Salmonellae (SHJ2037, B) carrying s.tGlmS+p were injected into CT26 tumor-bearing mice through the tail vein (3×107 CFU). Tumor tissues were sampled on the indicated days, homogenized, and spread on GlcNAc-supplemented LB plates containing kanamycin and chloramphenicol for the enumeration of total number of bacteria and ampicillin for the determination of plasmid-carrying bacteria.