Maintenance of <i><sup>s.t</sup>GlmS<sup>+</sup>p</i> in <i>S. typhimurium</i> proliferating in tumor tissue.

<p>(A) GlmS<sup>−</sup> mutant (SKS1002, A) and parental <i>Salmonellae</i> (SHJ2037, B) carrying <i><sup>s.t</sup>GlmS<sup>+</sup>p</i> were injected into CT26 tumor-bearing mice through the tail vein (3×10<sup>7</sup> CFU). Tumor tissues were sampled on the indicated days, homogenized, and spread on GlcNAc-supplemented LB plates containing kanamycin and chloramphenicol for the enumeration of total number of bacteria and ampicillin for the determination of plasmid-carrying bacteria.</p>