MOG-specific Th1 and Th17 cell differentiation.
A. T cells from 2D2 mice were activated under Th1 and Th17 polarizing conditions. Vα3.2 and Vβ11 transgenic TCR chains, as well as intracellular IL-17 and IFN-γ cytokine expression was assessed by FACS. Data shown are gated in the CD4+ population. B. IFN-γ and IL-17 cytokines from culture supernatants of Th1 and Th17 polarized cells were quantified by ELISA. C. IFN-γ, Tbet, IL-17 and RORγt gene expression was quantified by real-time PCR of Th1 and Th17 polarized cells. Data shown are representative (A) or a mean of a minimum of 5 experiments. Error bars indicate SEM (B and C).