Figure 1.TIF (2.23 MB)
MOG-specific Th1 and Th17 cell differentiation.
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posted on 2010-11-29, 01:41 authored by Helena S. Domingues, Marsilius Mues, Hans Lassmann, Hartmut Wekerle, Gurumoorthy KrishnamoorthyA. T cells from 2D2 mice were activated under Th1 and Th17 polarizing conditions. Vα3.2 and Vβ11 transgenic TCR chains, as well as intracellular IL-17 and IFN-γ cytokine expression was assessed by FACS. Data shown are gated in the CD4+ population. B. IFN-γ and IL-17 cytokines from culture supernatants of Th1 and Th17 polarized cells were quantified by ELISA. C. IFN-γ, Tbet, IL-17 and RORγt gene expression was quantified by real-time PCR of Th1 and Th17 polarized cells. Data shown are representative (A) or a mean of a minimum of 5 experiments. Error bars indicate SEM (B and C).
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EAETh 17 cellsTh 1 phenotypeTh 17 CellsT cell linesTh 1-like cellsTh 17-like cytokine signatureExperimental Autoimmune Encephalomyelitis BackgroundTCRTh 17 cells potentiatedTh 17 cells lysed autoantigenT cellsTh 17 lineagesFindings CD 4I-AIFN -γMOGTh 17 lesionsIL -17Th 1 lesionsTh 17 phenotypeTh 1 cellsCNSTh 17-like cellsTh 1myelin oligodendrocyte glycoprotein
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