MMIHC using cell blocks.

(A) Image of an MCF-7 cell block aligned with the MMIHC device. Scale bar, 500 µm. (B) Image processing DAB reaction. The cell block was stained at discrete sites with ER, HER2, PR, and Ki-67 antibodies. Scale bar, 500 µm. (C) Completed cell block slide after detachment from the platform. Samples were not damaged by the detachment process. Inset shows a magnified view. Scale bars, 3 mm. (D) Comparison of immunohistochemical staining for ER, HER2, PR, and Ki-67 using conventional IHC versus the MMIHC platform (1000×). The staining quality of biomarkers using the MMIHC platform was comparable to that of the conventional method.