MFG-E8 gene expression in the intestine.

Total RNA from gut tissues of Sham and WBI animals were extracted and reverse transcribed. The mRNA expression of MFG-E8 was determined by real-time PCR and fold change over GAPDH is shown. Data are presented as mean ± SE (n = 3–4) and compared by Student's-t-test. *P<0.05 vs. Sham.