<p><b>A</b> An optimal configuration for subsequent membrane-binding extracted from the ensemble of BD simulations conducted using an uncharged, zwitterionic lipid bilayer containing PI(3,4,5)P₃. In this case <i>r</i> = 9 Å, <i>d</i> = 18 Å and <i>θ</i> = 27°. <b>B</b> A snapshot at the end of a subsequent 100 ns MD simulation. <b>C, D</b> Fingerprint plots showing the location of PH domain residues contacting the phosphorus atoms of PI(3,4,5)P₃ in <b>C</b> the X-ray structure (PDB 1FGY) and <b>D</b> the 100 ns snapshot from the MD simulation shown in <b>B</b>. In each plot the minimum distances between each residue and each phosphorus atom are shown.</p>