Location of non-synonymous changes in the polyprotein of DENV-1 strains in relation to the reference sequence FGA/89.

Polar and non-polar amino acids are coded as follows: hydrophobic in pink (Ala, Phe, Ile, Leu, Met, Pro, Val, Trp); polar but uncharged in light blue (Cys, Gly, Asn, Gln, Ser, Thr, Tyr); negatively charged in gray (Asp, Glu); and positively charged in red (His, Lys, Arg).