Location and conservation of FGFR2 mutations. A) Schematic model of FGFR2 with bound ligand (FGF).

The locations of the novel p.K660N and p.R203C mutations are marked by red dots. TK1/2: tyrosine kinase domains 1 and 2; D1–3: immunoglobulin-like domains 1–3. B) Conservation of FGFR2 mutations. Arrows indicate localization of mutations. Above CLUSTALW alignment of vertebrate FGFR2s and human FGFRs. Below: ConSeq prediction. Amino acid conservation grade is colour-coded. The predicted status of each residue, buried (b) or exposed (e), is marked below the amino acid sequence. Slowly evolving and exposed residues are predicted to be functional (f), whereas slowly evolving and buried residues are predicted to be structurally important (s).