Localization of SIV-specific CD8low/− T cells in lymph nodes.
In each set of panels, the left panels show tetramer staining (red), the middle panels show CD8 staining (green), and the right panels merged images of the red and green stain (A) and (D) show Mamu-A*01 Tat SL8 staining and (F) shows staining from the negative control Mamu-A*01 FLP tetramer. Panels (D) and (E) are derived from (A) and (B), but show only the tetramer stained in order to more easily note the lack of CD8 staining on the cells in the cluster of cells in the upper right-hand quadrant of the image delineated by the purple line. These are representative images from axillary lymph nodes from SIV-infected rhesus macaque #27357 at 20 days post-infection. All images are confocal Z-scans collected using a 20× objective. Scale Bar = 50 microns.