Localization of Fur4-GFP under nitrogen starvation conditions.

(A) KNP74(ura4+), and KNP95 (Δura4) cells expressing Fur4-GFP were grown in YES liquid medium for 12 h. Cells were washed twice with sterile water and suspended in EMM, EMMU, EMM(-N), or EMMU(-N) media and incubated at 30°C for 1 h. Cells were observed using fluorescence microscopy. Bar: 10 μm.