Lnk mediates responses to oxidative stress and starvation and is required for metabolic regulation.

(A) Survival of male flies fed 5% hydrogen peroxide. Lnk mutant males live approximately 50% longer than controls and mutants carrying a Lnk genomic rescue (GR) construct (p<0.001). Median survival times are 2.6 days for w^Dah (black line; n = 97), 3.9 days for Lnk^Del29 (blue line; n = 93) and 2.6 days for LnkGR; Lnk^Del29/Lnk^Del29 (green line; n = 100). (B) Survival of female flies fed 5% hydrogen peroxide. Lnk mutant females live approximately 50% longer than controls and mutants carrying a Lnk genomic rescue (GR) construct (p<0.001). Median survival times are 3.1 days for w^Dah (black line; n = 93), 5.6 days for Lnk^Del29 (red line; n = 105) and 3.1 days for LnkGR; Lnk^Del29/Lnk^Del29 (green line; n = 100). (C) Survival of male flies under starvation conditions. Lnk mutant males live 30% longer than controls and mutants carrying a Lnk genomic rescue (GR) construct (p<0.001). Median survival times are as follows: 2.0 days for w^Dah (black line; n = 92), 2.6 days for Lnk^Del29 (blue line; n = 86) and 1.9 days for LnkGR; Lnk^Del29/Lnk^Del29(green line; n = 97). (D) Survival of female flies under starvation conditions. Lnk mutant females live 35% longer than controls and mutants carrying a Lnk genomic rescue (GR) construct (p<0.001). Median survival times are as follows: 4.3 days for w^Dah (black line; n = 94), 5.8 days for Lnk^Del29 (red line; n = 95) and 4.7 days for LnkGR; Lnk^Del29/Lnk^Del29 (green line; n = 95). (E) Whole-fly content of triglycerides (TAG) per mg of fly (fresh weight). Data are presented as means (n = 10) ± SEM. ** denotes statistically significant difference (p<0.05). (F) Whole-fly glycogen content per mg of fly (fresh weight). Data are presented as means (n = 10) ± SEM. ** denotes statistically significant difference (p<0.05). (G) Whole-fly trehalose content per mg of fly (fresh weight). Data are presented as means (n = 10) ± SEM. ** denotes statistically significant difference (p<0.05).