Lipid-reduced (LR) growth conditions affect lipid biogenesis in cancer cells.
HepG2, PC3M, HOP62 and T24 cells growing in normal or LR medium for 72 hours, were incubated for the last 4 hours with 14C-acetate. Cellular lipids were extracted and the incorporation of 14C in the cellular lipids was determined by scintillation counting. Scintillation counts were normalized for sample DNA content. Results are representative of three independent experiments. *Significantly different (*p≤0,05; **p≤0,01; ***p≤0,001), n.s. not significant (p>0,05).